Problems during sample preparation for automatic cell counting

The sample doesn’t flow into the chamber

Especially with new (unused) sample chambers you may encounter an issue with the sample not being completely pulled through by capillary forces. In this case, it is usually helpful to lightly tap the chamber on a fixed surface.

The Yeast Tends to Cluster

Things to try:

• Use lukewarm water, dilute the sample a bit more than you normally would, and stir/shake really well.
• Prepare a solution using 0.5 Molar EDTA, 0.5% H2SO4, and a micro scoop of Papain to blend with the yeast sample.

In either scenario, even if clusters persist, the detection process remains highly reliable. To enhance accuracy, capture more than five images when dealing with yeast strains prone to clustering.

Air bubbles in the chamber

Bubbles may form while the chamber is being filled (see the image below). This can often interfere with accurate measurement. In order to remove any bubble(s), the chamber should be rinsed out and then refilled.

Difficulties Cleaning the Slide

If you find that your slides are not easy to clean, we recommend using Mucasol for the cleaning process.

Follow these steps for effective cleaning:

1. Create a dilution of 2% Mucasol solution.
2. Load the chamber with the diluted solution using a syringe and allow it to incubate for 10 minutes.
3. After incubation, clean the chamber thoroughly with plenty of de-ionized water, pumping it slowly, as you would during regular cleaning.

This process should effectively remove any cells or debris that may be stuck in the chamber.

If this initial cleaning is not sufficient, you can consider extending the incubation time or using a 3% solution. Keep in mind that a 3% solution may reduce the overall lifespan of the chamber, but it can be a worthwhile solution if the chamber is otherwise unusable.

If you need further assistance, please contact us at [email protected].