Recipe: Alkaline Methylene Violet 0.01%

Alkaline methylene violet solution – 100 ml

Chemicals:

  • Methylene violet 3RAX (e.g. Roth)
  • 0.1M Glycine buffer (pH 10.6)
  • Distilled water

Procedure:

  • Dissolve 0.1g methylene violet in 100 ml distilled water (0.1% MV stock solution)
  • Mix 10 ml of the MV stock solution with 90 ml of the 0.1M glycine buffer. This solution is used for the viability staining.

0.1M Glycine buffer (pH 10.6) – 100 ml

Chemicals:

  • 1M NaOH solution (Merck)
  • Glycine (Sigma)
  • Distilled water

Procedure:

  • Dissolve 0.75 g glycine in 70 ml water
  • Adjust pH with the NaOH solution to 10.6
  • Fill volume to 100 ml with distilled water
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